A constant temperature centrifuge differs from other types of centrifuges because it allows for the sample to be kept at a constant temperature throughout the centrifugation process. This is especially important in applications where temperature-sensitive samples are involved, such as in molecular biology, biochemistry, and cell biology.
The constant temperature centrifuge combines the functions of a regular centrifuge with a specialized temperature control system, which allows for the sample to be kept at a specific temperature during the entire centrifugation process. This type of centrifuge is useful for several applications, including DNA extractions, protein purification, and cell culture protocols.
When selecting a constant temperature centrifuge, it's important to consider several factors. Capacity is one of the primary considerations, as this will determine the amount of sample that can be processed at once. It's also important to consider speed, as this will determine the force exerted on the sample during centrifugation.
Another important factor to consider when selecting a constant temperature centrifuge is the type of rotor that comes with it. There are several rotor types available, including fixed-angle, swinging-bucket, and vertical rotors, each with its own advantages and disadvantages. Fixed-angle rotors are ideal for pelleting or separating samples of similar density, whereas a swinging-bucket rotor is suitable for gradient centrifugation or for spinning samples of varying density.
The constant temperature control system is also an important consideration when selecting a centrifuge. Some centrifuges feature an internal cooling system, whereas others have an external cooling system. An internal cooling system is more efficient when working with very sensitive samples, as it allows for the sample to be cooled directly during the centrifugation process. An external cooling system is less efficient but is more suitable when working with larger sample volumes.
In summary, a constant temperature centrifuge is an essential tool in many molecular biology, biochemistry, and cell biology applications. When selecting a constant temperature centrifuge, it's important to consider several factors, including speed, capacity, rotor type, and temperature control system. If you're unsure which centrifuge is right for your application, consult with a specialist to ensure you choose the one that meets your specific requirements.